I Want to Fall in Love After Getting Reincarnated as the Heroine on the Verge of a Bad Ending, but My OP Older Brother Won’t Leave Me Alone!? - Chapter 164
Translated by: ShinigamiTori
Chapter 164 ? Side Story: A Sister and A Brother
During the lunch break, I excitedly headed towards the courtyard after borrowing a certain book from the library.
Since the weather is so nice, I plan to spend the remaining time reading on a bench under the trees.
“Ah, Rene?”
“Ah… Good afternoon, Mylene-sama!”
There stood Mylene-sama, Julius’s classmate and an incredibly beautiful woman. She was so stunning today that just catching a glimpse of her took my breath away.
Though we had known each other for a while, I feel like we’ve gotten quite close through preparing for the school festival.
With her position as a duke’s daughter and her stunning beauty, she always seemed out of reach, and I used to get nervous just greeting her. But now, thanks to Mylene-sama’s friendly and kind nature, we’ve been having pleasant conversations lately.
“What’s wrong? What are you doing here?”
“It’s such nice weather, so I thought I’d read outside.”
It seems that Mylene-sama was called out for a confession earlier and turned it down without hesitation. I’ve always wished I could say something like, ‘I was confessed to right here… Hehe.’
Eventually, Mylene-sama turns her gaze to the book in my hand.
“Oh, Rene, you’re reading a romance novel.”
“It’s a bit embarrassing, but I’ve always longed for this kind of romance…”
That’s right, today I was planning to read a romance novel. I wanted to learn about love.
“That’s nice. I don’t usually read things like this, so I’m curious.”
Somehow, I ended up reading the romance novel “The End of Love and Desire” together with Mylene-sama.
“Well then, I’ll read it out loud.”
“Yes!”
It felt like a storytelling session, and my heart was racing. Mylene-sama’s voice was beautiful as well. I felt compelled to pay her for this.
“What’s the matter? You’re smiling so brightly.”
“I’ve always looked up to you like an older sister, so this makes me happy.”
“You can become my sister anytime.”
She patted my head with a gentle “there, there,” and I couldn’t help but feel shy. Then, I happily continued reading the book with Mylene-sama.
“Oh my, that’s amazing.”
“W-Wow…”
The novel had a passionate kissing scene, and I couldn’t help but wonder if it’s really okay to have such content in the school library.
Reading this kind of thing with someone else made it quite embarrassing. However, unlike my flustered state, Mylene-sama continued reading boldly, looking genuinely intrigued.
“There are scenes between women too.”
“T-That’s true.”
A beautiful woman even appears and approaches the heroine, and the story takes a chaotic turn. Reading a story like this while being so close to a beautiful woman is really making my heart race.
“Your face is red. Is something wrong?”
“N-No…”
“Could it be that you’re conscious of it? I think I could be interested in someone as cute as you, Rene.”
“Eep!”
Mylene-sama smiled seductively and gently tilted my chin, bringing her stunningly beautiful face closer. Please wait!
A sweet, floral, and incredibly pleasant scent wafted toward me, causing my heart to race, and I squeezed my eyes shut tightly.
“Hey, can you not play around with Rene?”
Suddenly, I heard Julius’ voice, and I felt myself being pulled back. When I opened my eyes, I saw Julius holding me close with a slightly exasperated look on his face.
It looked like he had spotted us from afar and hurried over. Julius shot a brief glare at Mylene-sama and tightened his arms around me.
“Really, you have a twisted sense of humor. Talking nonsense like that when you’re not interested in women at all.”
“Because watching Rene all flustered is just too cute. Honestly, I prefer her over you.”
With a delighted smile, Mylene-sama rose to her feet and gently touched my cheek.
At the same time, I heard Julius clicking his tongue right by my ear.
“Let’s play again sometime.”
“Y-Yes…”
With that, Mylene-sama gracefully swept her long, beautiful blonde hair as she walked away.
So, she had just been teasing me for her own amusement, but here I was, feeling almost honored to have been “played with.” An overpowering beauty like hers is truly something.
Meanwhile, Julius let out a big sigh as he continued to hold me in his arms.
“Seriously annoying.”
“Huh?”
“Your face looks like you’re genuinely flustered.”
“No, no, that was just unavoidable!”
It’s impossible not to feel nervous when a beauty like that comes on to you, even if she’s the same sex.
Even his sulking face was a work of art, and as Julius brought his scowling face closer, my heart pounded without a break.
“You never make that kind of face for me.”
“T-That’s not true…”
Yes, recently, it’s been Julius who makes me feel this way. He doesn’t need to sulk about it. There’s no need for him to sulk over it.
“Really?”
“Yes! So, I’d prefer it if you could move back a little…”
Perhaps sensing the situation, Julius gave a charming smile.
“Oh, it’s true. Your face is all red, it’s cute.”
“…”
Those words made my heart race even more.
I think it’s really unfair of Julius to be able to say something like this so casually.
“S-So, how about we distance ourselves a bit now?”
“Only if you promise you won’t feel flustered about anyone but me.”
“T-That’s a bit…”
I don’t often get flustered about men, but it turns out I’m weak to beautiful women, so there’s no way I can make that promise.
Then, Julius said, “You traitor,” and, like Mylene-sama earlier, grabbed my chin.
“I don’t get flustered by anyone other than you, Rene. How cruel.”
“Anyone would look up to someone like Mylene-sama, a beautiful older sister.”
“Hey, isn’t it a bit too much to want both an older brother and an older sister?”
“I-I’m sorry…”
Realizing I was being greedy, I apologized, and Julius continued, “In that case…”
“What if I make Yoshida-kun my little brother and act all affectionate to him, huh? How would you feel about that?”
“Eh…?”
The unexpected question left me speechless.
From Julius’ perspective, it may have been a similar situation, but still, something like that??….
“To be honest, I really want to see that.”
“Huh?”
??After that, I was scolded fiercely and had to promise not to get too close to to either boys or girls.
On top of that, Julius went and tattled to Yoshida, and as a result, I couldn’t speak to Yoshida for a while—but that’s a whole other story.
Hi, I’m Tori, the translator of this novel! If you like the novel and would like to support me, feel free to click the button below to go to my ko-fi! (??`?) Please also consider sending some love by leaving a rating/review for this series on Novel Updates! Thank you ??
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L-Glutamine is well studied and some of the helpful amino acids
that exists. Its total results on the intestine include regulating the GI microbiome, bettering the functions of the intestines, and lowering constipation. In 1989, a study(2) explored the isolation and biological potential of the
KPV tripeptide. Researchers isolated the KPV peptide and offered it to the experimental mice
to evaluate its potential to mitigate ear swelling.
KPV’s potential anti-inflammatory motion may be induced by inactivating inflammatory pathways and presumably
inhibiting the synthesis and release of pro-inflammatory cytokines in intestinal and immune cells.
KPV exerts its results by binding to melanocortin receptors (MC1R and MC3R), which are concerned in inflammation regulation, immune
steadiness, and wound healing (Dinparastisaleh & Mirsaeidi).
The expression of pro-inflammatory cytokines is understood to be involved in intestinal
irritation. As expected, real-time RT-PCR experiments showed that DSS treatment increased mRNA
levels of various pro-inflammatory cytokines (IL-6, IL-12, IFN-?,
IL-1?) in mouse colon (Figure 7). Apparently, KPV remedy decreased the expression of
those cytokines and this effect was vital for IL-6 and IL-12 (Figure 7A, B).
Moreover, the incorporation of EGCG might considerably enhance the tissue adhesion properties of the PPP hydrogel at 37 °C.
PPP_2%E displayed an appropriate gelation temperature (36.2 °C), gelation time (100 s) and storage modulus (48 Pa). Tripeptide KPV as a mannequin drug was easily dissolved in chilly PPP_2%E precursor
answer to organize %E hydrogel. The anti-inflammatory activity and promotion of cell migration potential by KPV in PPP-2% E hydrogel had been nicely maintained.
Based on the mixed anti?inflammatory and antimicrobial results of some peptides, the
chance for infection could also be lower than with
conventional immunosuppressive agents. Furthermore, the broad anti?inflammatory impact will not end in robust immunosuppression as seen with the corticosteroids or systemic calcineurin inhibitors.
According to preliminary out there toxicity and security data, the opposed
results of ??MSH?related tripeptides seem to be minimal.
Given that KPV enters the nucleus, the next chance examined was that KPV would possibly favour
I?B? interplay with NF?B by blocking its phosphorylation by the IKK complicated or,
alternatively, blocking I?B? degradation. Coli BL21 cells by inducing
expression of 6h (20°C) with zero.2mM isopropyl-1-thio-?-D-galactopyranoside (IPTG).
Control lysates had been generated from parallel cultures by omitting the IPTG therapy and bacterial lysis was performed as described elsewhere [14].
Nunc Immunosorp plates were coated with 10?g.ml-1 of the in vitro-translated protein or the identical concentration of non-induced lysate protein overnight at 4°C.
Plates were then washed 3 times in Tris-buffered saline
containing zero.01% Tween 20 (TBST) before being blocked for 1h in 10?g.ml-1 BSA.
After an additional collection of washes, 1?g of protein obtained from HBE cells expressing FLAG-p65RelA was added to each well together with KPV
at concentrations starting from zero.01?30?g.ml-1. Every
nicely was then incubated with HRP-conjugated mouse
anti-FLAG IgG for 1h at room temperature followed by three ultimate washes in TBST.
Research applications could embody in vitro studies on intestine epithelial or
skin keratinocytes, animal fashions for IBD or wound healing, or blended peptide
research. Oath Peptides provides pure KPV
and innovative multi-peptide blends like KLOW specifically for research-centric settings.
Irritation is at the core of most gut-related problems, from irritable bowel syndrome (IBS) and inflammatory bowel illness (IBD) to leaky
gut and food sensitivities. KPV’s anti-inflammatory effects work by blocking the pro-inflammatory cytokines (like
TNF-? and IL-6) that drive tissue injury within the gut lining[1].
The result’s lowered inflammatory damage, better barrier operate, and improved microbiome stability.
KPV peptide is being studied for its effects on gastrointestinal
illnesses like ulcerative colitis, skin issues involving irritation, and even respiratory
irritation via its action on bronchial epithelial cells.
Still, researchers posit that the molecule might have one critical potential drawback in comparability with KPV—the potential for
an increase in melanin manufacturing and pigmentation along the
floor of the epidermal layer.
Its capacity to improve mucosal repair and facilitate wound therapeutic positions it
as a promising therapeutic solution for circumstances characterised by tissue injury.
In the pursuit of optimum well being and wellness, figuring out compounds that can naturally
support the physique’s complicated techniques is paramount.
This fascinating tripeptide, derived from the physique’s
own alpha-MSH (melanocyte-stimulating hormone), is quickly
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At NINGBO INNO PHARMCHEM CO.,LTD., we’re dedicated to providing high-quality peptide ingredients, and KPV is a prime example of a
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in Kpv peptide for skin repair is closely linked to its capacity to support cellular
regeneration and barrier perform.
Discussions usually focus on optimum analysis protocols,
delivery strategies, and potential functions in numerous inflammatory circumstances.
These community views serve as priceless indicators of research
tendencies and areas requiring further scientific investigation beneath proper laboratory circumstances.
Contemporary analysis applications for KPV peptide span a quantity of therapeutic areas, with investigators exploring novel approaches to inflammatory disease administration. Laboratory protocols have
evolved to include superior delivery methods and combination therapies that maximize therapeutic potential while minimizing opposed results.
Recent analysis group discussions spotlight the
peptide’s potential in addressing treatment-resistant inflammatory situations the place conventional therapies have shown limitations.
It just isn’t an FDA- or EMA-approved drug, and it can’t be marketed as a supplement or prescription therapy.
In short, KPV doesn’t just suppress inflammation like steroids do—it
resets the immune response, allowing therapeutic to
happen while nonetheless preserving normal protection features.
Think of KPV as the “business end” of ?-MSH—a compact piece carrying the peptide’s inflammation-fighting
functionality whereas stripping away functions not wanted for
therapeutic or analysis use. Interplay of DRAQ5 with herring sperm DNA (0.2mg.ml-1) within the presence of 0 (closed circles),
1 (grey squares) or 10 (grey circles) ?g.ml-1 KPV. Price of
improvement of DRAQ5 fluorescence within the nuclei of stay cells
was monitored in the presence of 0, 0.1, 1 or 10?g.ml-1 KPV included in the medium.
Pepsite analyses residue charge, hydrophobicity and protein construction to provide a statistical likelihood
of interplay between peptide and protein sequences. The analysis is compared to the probability that a random sequence
of residues will bind to the identical region.
A probability cut-off worth of 0.three was taken to characterize statistical significance.
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